Under what conditions can a genetically diverse population of organisms form 2 species over time?

1) isolation can be geographical, it arises as a result of the appearance of a physical barrier between parts of the population
2) isolation can be ecological, it occurs when a part of the population changes the ecological niche
3) isolation can lead to the formation of two species in the event that it is impossible to cross and exchange genes between new populations (reproductive isolation)

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