Reproductive isolation impedes the mixing of representatives of the species during sexual reproduction. It is provided by many mechanisms, for example, differences in ranges, different periods or places of reproduction, behavior in the mating season, and many others. Reproductive isolation is the main sign of the emergence of a new species.
Various populations of toads can serve as an example of reproductive isolation: some lay eggs in shallow reservoirs (puddles, streams), others in large ones (ponds, swamps), which excludes hybridization.
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