When decoding the maize genome, it was found that the proportion of nucleotides with thymine in a fragment of a DNA

When decoding the maize genome, it was found that the proportion of nucleotides with thymine in a fragment of a DNA molecule is 25%. Using Chargaff’s rule describing the quantitative relationships between different types of nitrogenous bases in DNA (G + T = A + C), calculate the amount (in%) in this sample of nucleotides with guanine.

Adenine (A) is complementary to thymine (T), and guanine (G) is complementary to cytosine (C), therefore the number of complementary nucleotides is the same; Chargaff’s rule: (A + T) + (G + C) = 100%
By condition T = 25%
H + C = 100% – (25% + 25%) = 50%
G = C = 50%: 2 = 25%

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